Since the offside rule was adopted in RoboCup-98; many teams without teamwork ability got offside penalty many times in their matches. Those teams who have dribble skill won; because most of other teams have not efficient defence strategy. In this paper; we consider an efficient teamwork-based defence method called line defence. To achieve the line defence; a set of basic teamwork abilities (including positioning;marking;notifying and avoiding) are proposed. We design five types of soccer players by combining those basic abilities in various ways. The simulation results showed that all of teamwork abilities are important to the line defence.